Epicuticular Wax as a Mechanical Barrier in Transmission of CLCuV : Role of Epicuticular Waxes in the Susceptibility of Gossypium Arboreum Cotton to CLCuV

Book Cover Epicuticular Wax as a Mechanical Barrier in Transmission of CLCuV : Role of Epicuticular Waxes in the Susceptibility of Gossypium Arboreum Cotton to CLCuV

Description

Epicuticular waxes plays very important role in hindering the attachment of insects to the leaves. It was hypothesized that wax act as mechanical barrier in transmission of CLCuV. Present book contain the study of the wax deficient mutant plants GaWM3 (50 % less wax than G. arboreum) were used as experimental plants while Gossypium arboreum (786) were used as negatively controlled plants as it is reported that whitefly can not transmit virus in it and G. hirsutum (MNH-93) were taken as negative and positive control plants because it has severe viral attack in past. After extracting DNA, RCA techniques was used to increase the quantity of CLCuV genome. For further conformation RCA product was used as template for CLCuV primer specific PCR. It was found that the G. hirsutum (MNH-93) was CLCuV positive and G. arboreum (786) was CLCuV negative. But most strikingly the wax deficient GaWM3 mutant plants which contain 50% less than G. arboreum (786) wax were CLCuV positive. Thus it is concluded that the wax act as a mechanical barrier in transmission of CLCuV and wax gene can be introduced to make CLCuV resistant crop.

Details

Author(s)
Muhammad Azmat Ullah Khan, Dr. Ahmed Ali Shahid, Dr. Adbul Qayyum Rao
Format
Paperback | 104 pages
Dimensions
150.11 x 219.96 x 6.1mm | 204.12g
Publication date
26 Oct 2013
Publisher
VDM Verlag Dr. Müller e.K.
Language
English
ISBN10
3639380657
ISBN13
9783639380651